Introduction

key concepts
  • Microbes, alternatively known as microorganisms, are essential to humans for processes such as digestion, waste excretion and nutrient absorption.
  • Some primary examples are bacteria (e.g amoeba) and fungi (e.g yeast)

Bacteria Growth

  • Bacteria require suitable temperature, pH and nutrients provisions to grow (oxygen and water)
    • Temperature and pH - enzymes in bacteria can denature.
    • Oxygen and water are needed for growth.
  • However, growth is limited as resources (nutrients) are limited, or the bacteria growth can no longer be supported by the environment (too small environment, too much waste etc.)

Inhibiting Bacteria Growth

  • Hygienic practises such as sanitisation (disinfectant) and personal hygiene (washing hands, antiseptics), cleaning equipment, and appropriate food storage (refrigeration, use-by dates) as well as avoiding cross-contamination prevent food spoilage via microbes.

The Importance of Microorganisms

  • Microorganisms release enzymes that enable sugar digestion, and can also inhibit pathogenic bacteria - they are quite common in the digestive system.
  • Recombinant DNA technology:: AKA genetic modification. Uses plasmids from bacteria as they are cheap, easy to obtain and rapidly growing in order to produce things such as human insulin.
    • Requires the identification of a suitable plasmid from a bacteria using a restriction enzyme.
    • Then, needs a specific gene that is to be replicated (from another cell) to be isolated. The same restriction enzyme is used to cut this.
    • Ligase enzyme is used to replace the gene from the other cell in the bacteria, and the plasmid is returned to the original bacteria.